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  Principal Investigators
LI Wei
Developmental Biology
+86-10-64807297  /  +86-10-64807306
The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Building of Institute for Stem Cell and Regeneration, CAS, Yard A 3, Datun Road, Chaoyang District, Beijing 100101, P.R.China
Group of Stem Cells and Cell Cycle Regulation      

2006-2012: Ph.D. in Developmental Biology, Institute of Zoology, CAS / University of Chinese Academy of Sciences
2002-2006: B.Sc. in Life Sciences, College of Life Sciences, Wuhan University

2013-2015: Principle Investigator of Star Program, Group leader of Stem Cell and Cell Cycle Regulation, Institute of Zoology, CAS
2016-Ongoing: Professor, Principle Investigator, Group leader of Stem Cell and Cell Cycle Regulation, Institute of Zoology, CAS
2017.09-Ongoing: Vice Director of State Key Laboratory of Stem Cell and Reproductive Biology

Research Interests:

An old but still unaccomplished goal in biology is to elucidate which and how, genes and their regulations, determine a phenotype/function. Although a series of landmark achievements have emerged in answering this question, it is still very challenging to reconstruct a phenotype/function completely using designed genetic modules. The long-term goal of my research is to understand the genetic basis of certain reproduction- and regeneration-related functions/phenotypes, and to reconstitute such phenotype/function in a given cell or organism for research and therapeutic purposes. Current interests in my laboratory include: (i). developing cutting-edge genome engineering technologies and mammalian synthetic biology. (ii). dissection of genetic and epigenetic basis of reproduction- and regeneration-related phenotypes. (iii) clinical application of gene therapy.

Awards and Honors:

2020: Outstanding Science and Technology Achievement Prize of the CAS, 3rd Contributor
2019: Young Affiliate of TWAS (The World Academy of Sciences for the advancement of science in developing countries)
2018: Young Scientists Award of the CAS
2017: Top Ten Outstanding Youth of the CAS
2015: LU JIAXI Young Talents Award
2014: Recipient of the Excellent Young Scientists Fund of the National Natural Science Foundation of China
2013: International Award of the Japanese Association for Laboratory Animal Science
2013: Outstanding Science and Technology Achievement Prize of the CAS, 3rd Contributor

Professional Activities:

Research Grants:

Selected Publications:
(*corresponding author)
  1. Guo L#, Sun X#, Wang X#, Liang C#, Jiang H#, Gao Q#, Dai M#, Qu B, Fang S, Mao Y, Chen Y, Feng G, Gu Q, Wang RR*, Zhou Q*, Li W*. SARS-CoV-2 detection with CRISPR diagnostics. (2020) Cell Discov. 6:34. doi: 10.1038/s41421-020-0174-y.
  2. Wang LY#, Li ZK#, Wang LB#, Liu C#, Sun XH, Feng GH, Wang JQ, Li YF, Qiao LY, Nie H, Jiang LY, Sun H, Xie YL, Ma SN, Wan HF, Lu FL*, Li W*, Zhou Q*. Overcoming Intrinsic H3K27me3 Imprinting Barriers Improves Post-implantation Development after Somatic Cell Nuclear Transfer. (2020) Cell Stem Cell 27(2):315-325.e5. doi: 10.1016/j.stem.2020.05.014.
  3. Qu B#, Wu S#, Jiao G#, Zou X#, Li Z#, Guo L#, Sun X, Huang C, Sun Z, Zhang Y, Li H, Zhou Q, Sui R*, Li W*. Treating Bietti crystalline dystrophy in a high-fat diet-exacerbated murine model using gene therapy. (2020) Gene Ther. 27(7-8):370-382.doi: 10.1038/s41434-020-0159-3.
  4. Mao J#, Xu K#, Han J#, Feng G, Zhang Y*, Li W*. Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon. (2020) Protein Cell. 11(6):452-457. doi: 10.1007/s13238-020-00702-0.
  5. Teng F#, Guo L#, Cui T, Wang XG, Xu K, Gao Q, Zhou Q*, Li W*. CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity. (2019) Genome Biology 20(1):132. doi: 10.1186/s13059-019-1742-z.
  6. Teng F#, Li J#, Cui T#, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou Q*, Li W*. Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. (2019) Genome Biology 20(1):15. doi: 10.1186/s13059-019-1620-8.
  7. Li Y#, Wang L#, Zhang L#, He Z#, Feng G, Sun H, Wang J, Li Z, Liu C, Han J, Mao J, Li P, Yuan X, Jiang L, Zhang Y, Zhou Q*, Li W*. Cyclin B3 is required for metaphase to anaphase transition in oocyte meiosis I. (2019) Journal of Cell Biolology 218(5):1553-1563. doi: 10.1083/jcb.201808088.
  8. Wang J#, Wang L#, Feng G#, Wang Y#, Li Y, Li X, Liu C, Jiao G, Huang C, Shi J, Zhou T, Chen Q, Liu Z, Li W*, Zhou Q*. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. (2018) Cell 175(7):1887-1901. doi: 10.1016/j.cell.2018.11.039.
  9. Teng F#, Cui T#, Feng G, Guo L, Xu K, Gao Q, Li T, Li J, Zhou Q, Li W*. Repurposing CRISPR-Cas12b for mammalian genome engineering. (2018) Cell Discovery 4:63. doi: 10.1038/s41421-018-0069-3.
  10. Zhang W#, Wan H#, Feng G#, Qu J#, Wang J, Jing Y, Ren R, Liu Z, Zhang L, Chen Z, Wang S, Zhao Y, Wang Z, Yuan Y, Zhou Q, Li W*, Liu G*, Hu B*. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys. (2018) Nature 560(7720):661-665. doi: 10.1038/s41586-018-0437-z.
  11. Li ZK#, Wang LY#, Wang LB#, Feng GH#, Yuan XW#, Liu C, Xu K, Li YH, Wan HF, Zhang Y, Li YF, Li X, Li W*, Zhou Q*, Hu BY*. Generation of Bimaternal and Bipaternal Mice from Hypomethylated Haploid ESCs with Imprinting Region Deletions. (2018) Cell Stem Cell 23(5):665-676.e4. doi: 10.1016/j.stem.2018.09.004.
  12. Wang X#, Li T#, Cui T#, Yu D#, Liu C#, Jiang L, Feng G, Wang L, Fu R, Zhang X, Hao J, Wang Y, Wang L, Zhou Q*, Li W*, Hu B*. Human embryonic stem cells contribute to embryonic and extraembryonic lineages in mouse embryos upon inhibition of apoptosis. (2018) Cell Research 28(1):126-129. doi: 10.1038/cr.2017.138.
  13. Xu K#, Yang Y#, Feng GH#, Sun BF#, Chen JQ#, Li YF, Chen YS, Zhang XX, Wang CX, Jiang LY, Liu C, Zhang ZY, Wang XJ, Zhou Q*, Yang YG*, Li W*. Mettl3-mediated m6A regulates spermatogonial differentiation and meiosis initiation. (2017) Cell Research 27(9):1100-1114. doi: 10.1038/cr.2017.100. (Cover story)
  14. Li W*, Zhou Q. New stem cell types for versatile applications. (2017) National Science Review 4(4):528-530. doi.org/10.1093/nsr/nwx054.
  15. Li X#, Cui XL#, Wang JQ#, Wang YK, Li YF, Wang LY, Wan HF, Li TD, Feng GH, Shuai L, Li ZK, Gu Q, Hao J, Wang L, Zhao XY, Liu ZH, Wang XJ, Li W*, Zhou Q*. Generation and application of mouse-rat allodiploid embryonic stem cells. (2016) Cell 164(1-2):279-292. doi: 10.1016/j.cell.2015.11.035.
  16. Wan HF#, Feng CJ#, Teng F#, Yang SH#, Hu BY, Niu YY, Xiang P, Fang WZ, Ji WZ, Li W*, Zhao XY*, Zhou Q*. One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system. (2015) Cell Research 25(2):258-261. doi: 10.1038/cr.2014.158.
  17. Li W#, Li X#, Li TD#, Jiang MG#, Wan HF, Luo GZ, Feng CJ, Cui XL, Teng F, Yuan Y, Zhou Q, Gu Q, Shuai L, Sha JH, Xiao YM, Wang L, Liu ZH, Wang XJ, Zhao XY, Zhou Q*. Genetic modification and screening in rat using haploid embryonic stem cells. (2014) Cell Stem Cell 14(3):404-414. doi: 10.1016/j.stem.2013.11.016.
  18. Li W#, Teng F#, Li T, Zhou Q*. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. (2013) Nature Biotechnology 31(8):684-686. doi: 10.1038/nbt.2652.
  19. Li W#, Shuai L#, Wan H#, Dong M#, Wang M, Sang L, Feng C, Luo GZ, Li T, Li X, Wang L, Zheng QY, Sheng C, Wu HJ, Liu Z, Liu L, Wang L, Wang XJ, Zhao XY*, Zhou Q*. Androgenetic haploid embryonic stem cells produce live transgenic mice. (2012) Nature 490(7420):407-411. doi: 10.1038/nature11435.
  20. Zhao XY#, Li W#, Lv Z#, Liu L, Tong M, Hai T, Hao J, Guo CL, Ma QW, Wang L, Zeng F*, Zhou Q*. iPS cells produce viable mice through tetraploid complementation. (2009) Nature 461(7260):86-90. doi: 10.1038/nature08267.