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论文题目: Multimode Drug Inducible CRISPR/Cas9 Devices for Transcriptional Activation and Genome Editing
论文题目英文:
作者: 卢佳①,赵晨①,赵迎泽①,张竞方①,张月,陈丽,Qi-Yuan Han,Yue Ying,Shuai Peng,艾润娜,王宇*
论文出处:
年: 2018
卷: 46
期: 5
页: Article No.e25
联系作者: 王宇
发表期刊: Nucleic Acids Research
ISSN:
第一作者所在部门:
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论文链接: https://academic.oup.com/nar/article/46/5/e25/4716932
影响因子:
摘要: Precise investigation and manipulation of dynamic biological processes often requires molecular modulation in a controlled inducible manner. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) has emerged as a versatile tool for targeted gene editing and transcriptional programming. Here, we designed and vigorously optimized a series of Hybrid drug Inducible CRISPR/Cas9 Technologies (HIT) for transcriptional activation by grafting a mutated human estrogen receptor (ERT2) to multiple CRISPR/Cas9 systems, which renders them 4-hydroxytamoxifen (4-OHT) inducible for the access of genome. Further, extra functionality of simultaneous genome editing was achieved with one device we named HIT2. Optimized terminal devices herein delivered advantageous performances in comparison with several existing designs. They exerted selective, titratable, rapid and reversible response to drug induction. In addition, these designs were successfully adapted to an orthogonal Cas9. HIT systems developed in this study can be applied for controlled modulation of potentially any genomic loci in multiple modes.
英文摘要:
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