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论文题目: Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation
论文题目英文:
作者: 赵晨①,张月①,赵迎泽,Yue Ying,艾润娜,张竞方,王宇*
论文出处:
年: 2018
卷: 13
期: 3
页: 609-617
联系作者: 王宇
发表期刊: ACS Chemical Biology
ISSN:
第一作者所在部门:
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论文链接: https://pubs.acs.org/doi/10.1021/acschembio.7b00606
影响因子:
摘要: Inducible modulation is often required for precise investigations and manipulations of dynamic biological processes. Transcription activator-like effectors (TALEs) provide a powerful tool for targeted gene editing and transcriptional programming. We designed a series of chemical inducible systems by coupling TALEs with a mutated human estrogen receptor (ERT2), which renders them 4-hydroxyl-tamoxifen (4-OHT) inducible for access of the genome. Chemical inducible genome editing was achieved via fusing two tandem ERT2 domains to customized transcription activator-like effector nuclease (TALEN), which we termed "Hybrid Inducible Technology" (HIT-TALEN). Those for transcription activation were vigorously optimized using multiple construct designs. Most efficient drug induction for endogenous gene activation was accomplished with minimal background activity using an optimized inducible TALE based SunTag system (HIT-TALE-SunTag). The HIT-SunTag system is rapid, tunable, selective to 4-OHT over an endogenous ligand, and reversible in drug induced transcriptional activation. Versatile systems developed in this study can be easily applied for editing and transcriptional programming of potentially any genomic loci in a tight and effective chemical inducible fashion.
英文摘要:
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