| 论文题目： | A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish |
| 论文题目英文： | |
| 作者： | Jun Xia, Zhixin Kang, Yuanyuan Xue, Yanyan Ding, Suwei Gao, Yifan Zhang, Peng Lv, Xinyu Wang, Dongyuan Ma, Lu Wang, Jing-Dong J. Han, and Feng Liu |
| 论文出处： | |
| 年： | 2021 |
| 卷： | Vol. 118 |
| 期： | 14 |
| 页： | 1-12 |
| 联系作者： | 刘峰 |
| 发表期刊： | PNAS |
| ISSN： | |
| 第一作者所在部门： | |
| 收录类别： | |
| 论文连接 | https://doi.org/10.1073/pnas.2015748118 |
| 影响因子： | |
| 摘要: | During vertebrate embryogenesis, fetal hematopoietic stem and progenitor cells (HSPCs) exhibit expansion and differentiation properties in a supportive hematopoietic niche. To profile the developmental landscape of fetal HSPCs and their local niche, here, using single-cell RNA-sequencing, we deciphered a dynamic atlas covering 28,777 cells and 9 major cell types (23 clusters) of zebrafish caudal hematopoietic tissue (CHT). We characterized four heterogeneous HSPCs with distinct lineage priming and metabolic gene signatures. Furthermore, we investigated the regulatory mechanism of CHT niche components for HSPC development, with a focus on the transcription factors and ligand–receptor networks involved in HSPC expansion. Importantly, we identified an endothelial cell-specific G protein–coupled receptor 182, followed by in vivo and in vitro functional validation of its evolutionally conserved role in supporting HSPC expansion in zebrafish and mice. Finally, comparison between zebrafish CHT and human fetal liver highlighted the conservation and divergence across evolution. These findings enhance our understanding of the regulatory mechanism underlying hematopoietic niche for HSPC expansion in vivo and provide insights into improving protocols for HSPC expansion in vitro. |
| 英文摘要： | |
| 外单位作者单位： |