姓  名: 李 卫
学  科: 发育生物学
电话/传真: +86-10-64807529 / 
电子邮件: leways@ioz.ac.cn
通讯地址: 北京市朝阳区大屯路
中国科学院动物研究所, 干细胞与生殖生物学国家重点实验室 100101
更多信息: 蛋白质修饰与配子发生研究组     

简历介绍:

  现为中国科学院动物研究所干细胞与生殖生物学国家重点实验室研究员,蛋白质修饰与配子发生研究组组长,科技部”中青年科技创新领军人才”,国家重点研发计划、自然科学基金重大研究计划重点项目和杰出青年基金项目负责人。1998年和2003年于兰州大学获得学士及博士学位。2003年8月至2009年5月分别在清华大学生物科学与技术系和美国国立健康研究院NIDDK从事博士后研究。李卫研究员主要从事生育力维持与重塑方面的研究,从配子成熟、减数分裂、体细胞-生殖细胞互作和干细胞维持与分化四方面系统深入地解析了不孕不育的发病机理,从而为生殖和衰老相关疾病的诊治提供新的靶点和思路。迄今已在Nature、Adv Sci、Nat Commun、J Cell Biol、Am J Hum Genet、EMBO J、PNAS、Cell Res、Nucleic Acids Res、Autophagy、Cell Death Differ、EMBO Rep、eLife等杂志发表论文80余篇。

研究领域:

1)配子发生尤其是减数分裂的翻译后修饰调控。
2)组蛋白修饰在配子发生以及干细胞维持和分化中的功能和作用机制。
3)生殖衰老相关疾病的分子基础。
4)生育力维持与重塑的新方法和新途径。

社会任职:

  • 2019 年-至今 动物学会生殖生物学委员会 常务委员
  • 2018 年-至今 细胞生物学会细胞器生物学分会 秘书长
  • 2017 年-至今 中国妇幼保健协会生育力保存专业委员会 常委
  • 2017 年-至今 生物物理学会衰老生物学分会 理事
  • 2015 年-至今《Sci Rep》编委
  • 2015 年-至今 生理学会生殖科学委员会 理事
  • 2015 年-至今 细胞生物学会教学和科普委员会 委员
  • 2013 年-至今 微生物学会模式真菌委员会 理事

获奖及荣誉:

承担科研项目情况:

  1. 国家杰出青年科学基金项目-男性不育致病机理(81925015,项目负责人;
  2. 中国科学院A类战略性先导科技专项-生殖器官体外再造及生命孕育(XDA16020701),子课题负责人;
  3. 国家重点研发计划“蛋白质机器与生命过程调控”重点专项-RNA 结合蛋白在精子发生过程中的作用机理(2016YFA0500900),项目负责人;
  4. 国家自然科学基金重大研究计划重点项目-自噬在迟发性性腺机能减退发生过程中功能和作用机制的研究(91649202),项目负责人;
  5. 国家自然科学基金重大研究计划集成项目-H2B泛素化在细胞重编程过程中的作用机制研究(91519317),项目负责人;
  6. 国家自然科学基金面上项目-端粒重复序列结合因子(Trf1)在减数分裂花束期形成过程中的作用机制(31471277),项目负责人;
  7. 国家重点基础研究发展计划“973”项目-雌性生殖细胞减数分裂的分子基础(2012CB944404),研究骨干;
  8. 国家自然科学基金面上项目- H2B泛素化在减数分裂过程中的作用机制(31171374),项目负责人;
  9. 国家重点基础研究发展计划“973”项目-精子遗传信息稳定传递的分子机理(2011CB944303),课题负责人;
  10. 国家自然科学基金项目-Ube2g2/gp78合成多聚泛素链的内在机制(30970603),项目负责人;

代表论著:

  1. Liu C#, Zhao HC#, Xiao S#, Han T, Chen Y, Wang T, Ma Y, Gao H, Xie Z, Du L, Li J, Li G*, Li W*. Slx5p-Slx8p promotes accurate chromosome segregation by mediating the degradation of synaptonemal complex components during meiosis. Adv Sci (Weinh). 2020 Jan 1;7(4):1900739. doi: 10.1002/advs.201900739.
  2. Gao H#, Khawar MB#, Li W*. Essential role of autophagy in resource allocation during sexual reproduction. Autophagy. 2020 Jan;16(1):18-27. doi: 10.1080/15548627.2019.1628543. Epub 2019 Jun 16.
  3. Wu B, Gao H, Liu C, Li W*. The coupling apparatus of the sperm head and tail. Biol Reprod. 2020 Jan 29. pii: ioaa016. doi: 10.1093/biolre/ioaa016.
  4. Yuan Y, Miao Y, Qian L, Zhang Y, Liu C, Liu J, Zuo Y, Feng Q, Guo T, Zhang L, Chen X, Jin L, Huang F, Zhang H, Zhang W, Li W, Xu G, Zheng H. Targeting UBE4A Revives Viperin Protein in Epithelium to Enhance Host Antiviral Defense. Mol Cell. 2020 Feb 20;77(4):734-747.e7. doi: 10.1016/j.molcel.2019.11.003. Epub 2019 Dec 4.
  5. Gao H, Khawar MB, Li W*. Autophagy in Reproduction. Adv Exp Med Biol. 2019; 1206:453-468. doi: 10.1007/978-981-15-0602-4_21.
  6. Khawar MB, Gao H, Li W*. Autophagy and Lipid Metabolism. Adv Exp Med Biol. 2019; 1206:359-374. doi: 10.1007/978-981-15-0602-4_17.
  7. Hua R#, Wei H#, Liu C#, Zhang Y, Liu S, Guo Y, Cui Y, Zhang X, Guo X, Li W*, Liu M*. FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis. Nucleic Acids Res. 2019, Vol. 47, No. 22 11755-11770
  8. Wang T, Gao H, Li W* and Liu C. Essential Role of Histone Replacement and Modifications in Male Fertility. Front. Genet. 2019 Oct 8;10:962. doi: 10.3389/fgene.2019.00962.
  9. Khawar MB, Gao H and Li W* Mechanism of Acrosome Biogenesis in Mammals. Front. Cell Dev. Biol. 2019, Sep 18;7:195. doi: 10.3389/fcell.2019.00195.
  10. Li M, Huang T, Li MJ, Zhang CX, Yu XC, Yin YY, Liu C, Wang X, Feng HW, Zhang T, Liu MF, Han CS, Lu G, Li W, Ma JL, Chen ZJ, Liu HB*, Liu K*. The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019 Aug 14;5(8): eaax1101. doi: 10.1126/sciadv.aax1101.
  11. Gu H#, Shi X#, Liu C#, Wang C, Sui N, Zhao Y, Gong J, Wang F, Zhang H, Li W*, Zhao T*. USP8 maintains embryonic stem cell stemness via deubiquitination of EPG5. Nat Commun. 2019 Apr 1;10(1):1465. doi: 10.1038/s41467-019-09430-4.
  12. Chai Q, Wang X, Qiang L, Zhang Y, Ge P, Lu Z, Zhong Y, Li B, Wang J, Zhang L, Zhou D, Li W, Dong W, Pang Y, Gao GF*, Liu CH*. A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy. Nat Commun. 2019 Apr 29;10(1):1973. doi: 10.1038/s41467-019-09955-8.
  13. Shang YL#, Zhu FX#, Yan J, Chen L, Tang WH, Xiao S, Mo WK, Zhang ZG, He XJ, Qiao J, Cao YX*, Li W*. Novel DPY19L2 variants in globozoospermic patients and the overcoming this male infertility. Asian J Androl. 2019 Mar-Apr; 21(2):183-189. doi: 10.4103/aja.aja_79_18.
  14. Zhu F#, Liu C#, Wang F#, Yang X#, Zhang J, Wu H, Zhang Z, He X, Zhang Z, Zhou P, Wei Z, Shang Y, Wang L, Zhang R, Ouyang YC, Sun QY, Cao Y*, Li W*. Mutations in PMFBP1 Cause Acephalic Spermatozoa Syndrome. Am J Hum Genet. 2018 Aug 2;103(2):188-199. 
  15. Yang L#, Liu C#, Zhao W#, He C, Ding J, Dai R, Xu K, Xiao L, Luo L, Liu S, Li W*, Meng H*. Impaired autophagy in intestinal epithelial cells alters gut microbiota and host immune responses. Appl Environ Microbiol. 2018 Aug 31;84(18). pii: e00880-18. doi: 10.1128/AEM.00880-18.
  16. Gao F#, Li G#, Liu C#, Gao H#, Wang H, Liu W, Chen M, Shang Y, Wang L, Shi J, Xia W, Jiao J, Gao F, Li J, Chen L*, Li W*. Autophagy regulates testosterone synthesis by facilitating cholesterol uptake in the Leydig cells. J Cell Biol. 2018 Jun 4;217(6):2103-2119. doi: 10.1083/jcb.201710078.
  17. Baranes-Bachar K, Levy-Barda A, Oehler J, Reid DA, Soria-Bretones I, Voss TC, Chung D, Park Y, Liu C, Yoon JB, Li W, Dellaire G, Misteli T, Huertas P, Rothenberg E, Ramadan K, Ziv Y, Shiloh Y. The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair. Mol Cell. 2018 Mar 1;69(5):866-878.e7. doi: 10.1016/j.molcel.2018.02.002.
  18. Gao H, Liu C, Li W*. Assessing Autophagy in the Leydig Cells. Methods Mol Biol. 2018 Feb 27. doi: 10.1007/7651_2018_123.
  19. Liu C, Khan J, Li W*. Assessing Autophagy in Sertoli Cells. Methods Mol Biol. 2018;1748:113-128. doi: 10.1007/978-1-4939-7698-0_10.
  20. Wang L#, Tu Z#, Liu C#, Liu H, Kaldis P, Chen Z*, Li W*. Dual roles of TRF1 in tethering telomeres to the nuclear envelope and protecting them from fusion during meiosis. Cell Death Differ. 2018 Jan 8. doi: 10.1038/s41418-017-0037-8.
  21. Liu C, Ma Y, Shang Y, Huo R*, Li W*. Post-translational regulation of the maternal-to-zygotic transition. Cell Mol Life Sci. 2018 Feb 9. doi: 10.1007/s00018-018-2750-y.
  22. Zhao H#, Wang Q#, Liu C, Shang Y, Wen F, Wang F, Liu W, Xiao W, Li W*. A Role for Respiration in Regulating Meiosis Initiation in Saccharomyces cerevisiae. Genetics. 2018 Mar;208(3):1181-1194. doi: 10.1534/genetics.118.300689.
  23. Shang Y, Yan J, Tang W, Liu C, Xiao S, Guo Y, Yuan L, Chen L, Jiang H, Guo X, Qiao J, Li W*. Mechanistic insights into acephalic spermatozoa syndrome-associated mutations in the human SUN5 gene. J Biol Chem. 2018 Feb 16;293(7):2395-2407. doi: 10.1074/jbc.RA117.000861.
  24. Liang Q, Xia W, Li W, Jiao J. RNF20 controls astrocytic differentiation through epigenetic regulation of STAT3 in the developing brain. Cell Death Differ. 2018 Feb;25(2):294-306. doi: 10.1038/cdd.2017.157.
  25. Shang Y, Zhu F, Wang L, Ouyang YC, Dong MZ, Liu C, Zhao H, Cui X, Ma D, Zhang Z, Yang X, Guo Y, Liu F, Yuan L, Gao F*, Guo X*, Sun QY*, Cao Y*, Li W*. Essential role for SUN5 in anchoring sperm head to the tail. Elife. 2017 Sep 25;6. pii: e28199. doi: 10.7554/eLife.28199.
  26. Wang L, Xu Z, Khawar MB, Liu C, Li W*. The histone codes for meiosis. Reproduction. 2017 Sep;154(3): R65-R79. doi: 10.1530/REP-17-0153.
  27. Wang L, Cao C, Wang F, Zhao J, Li W*. H2B ubiquitination: Conserved molecular mechanism, diverse physiologic functions of the E3 ligase during meiosis. Nucleus. 2017 Sep 3;8(5):461-468. doi: 10.1080/19491034.2017.1330237.
  28. Gao H, Li W*. The day after the 7th day of the Creation: Breakthrough of human embryo in vitro culture. Sci China Life Sci. 2017 Jun;60(6):665-667. doi: 10.1007/s11427-017-9023-y.
  29. Liu C, Liu W, Ye Y, Li W*. Ufd2p synthesizes branched ubiquitin chains to promote the degradation of proteins modified with atypical ubiquitin chains. Nat Commun. 2017 Feb 6;8:14274. doi: 10.1038/ncomms14274.
  30. Yang Y, Zhou C, Wang Y, Liu W, Liu C, Wang L, Liu Y, Shang Y, Li M, Zhou S, Wang Y, Zeng W, Zhou J, Huo R*, Li W*. The E3 ubiquitin ligase RNF114 and TAB1 degradation are required for maternal-to-zygotic transition. EMBO Rep. 2017 Feb;18(2):205-216. 
  31. Liu C, Song Z, Wang L, Yu H, Liu W, Shang Y, Xu Z, Zhao H, Gao F, Wen J, Zhao L, Gui Y, Jiao J, Gao F, Li W*. Sirt1 regulates acrosome biogenesis by modulating autophagic flux during spermiogenesis in mice. Development. 2017 Feb 1;144(3):441-451. 
  32. Yin H, Zheng L, Liu W, Zhang D, Li W*, Yuan L*. Rootletin prevents Cep68 from VHL-mediated proteasomal degradation to maintain centrosome cohesion. BBA - Mol Cell Res. 2017 Jan 13;1864(4):645-654.
  33. Xu Z, Song Z, Li G, Tu H, Liu W, Liu Y, Wang P, Wang Y, Cui X, Liu C, Shang Y, de Rooij DG, Gao F, Li W*. H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation. Nucleic Acids Res.  2016 Nov 16;44(20):9681-9697.
  34. Liu Y, Liu C, Dong W*, Li W*. Physiological functions and clinical implications of the N-end rule pathway. Front Med. 2016 Sep;10(3):258-70. doi: 10.1007/s11684-016-0458-7.
  35. Li S*, Liu C, Gu L, Wang L, Shang Y, Liu Q, Wan J, Shi J, Wang F, Xu Z, Ji G*, Li W*. Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36. Open Biol. 2016 Aug;6(8). pii: 160177. doi: 10.1098/rsob.160177.
  36. Shang YL, Wang HN, Jia PF, Zhao HC, Liu C, Liu WX, Song ZH, Xu ZL,Yang L, Wang YF, Li W*. Autophagy regulates spermatid differentiation via degradation of PDLIM1. Autophagy. 2016 Sep;12(9):1575-92. doi: 10.1080/15548627.2016.1192750.
  37. Gao F, Shang Y, Liu W, Li W*. The linkage specificity determination of Ube2g2-gp78 mediated polyubiquitination. Biochem Biophys Res Commun. 2016 May 13;473(4):1139-43.
  38. Liu YJ, Liu C, Chang ZN, Wadas B, Brower CS, Song ZH, Xu ZL, Shang YL, Liu WX, Wang LN, Dong W*, Varshavsky A*, Hu RG*, and Li W*, Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway. J Biol Chem. 2016 Apr 1;291(14):7426-38.
  39. Wen FP, Guo YS, Hu Y, Wang YT, Wang Q, Yu HY, Tang CM, Yang J, Zhou T, Sha JH, Guo XJ*, Li W*. Distinct temporal requirements for autophagy and proteasome in yeast meiosis. Autophagy. 2016 Apr 2;12(4):671-88.
  40. Liu C,Wang HN, Shang YL, Liu WX, Song ZH, Zhao HC, Wang LN, Jia PF, Gao FY, Xu ZL, Yang L, Gao F, Li W*. Autophagy is required for ectoplasmic specialization assembly in Sertoli cells. Autophagy. 2016 May 3;12(5):814-32.
  41. Klionsky DJ,...Li W... Zughaier SM. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 2016 Jan 2;12(1):1-222.(8.593)
  42. Liu C, Li W*, The molecular mechanism underlying acrosome biogenesis. Spermatogenesis: Molecular Mechanisms, Regulation and Biological Perspectives. 2016, pp. 1-30 (Nova Science Publishers).
  43. Liu M, Cao GM, Zhang YM, Qu JP, Li W, Wan XR, Li YX, Zhang ZB*, Wang YL*, Gao F*. Changes in the morphology and protein expression of germ cells and Sertoli cells in plateau pikas testes during non-breeding season. Sci Rep. 2016 Mar 4;6:22697. doi: 10.1038/srep22697.
  44. Zhang P, He Q, Chen D, Liu W, Wang L, Zhang C, Ma D, Li W, Liu B, Liu F. G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition. Cell Res. 2015 Oct;25(10):1093-107.
  45. Wang Q, Liu C, Tang C, Guo H, Liu Y, Wang L, Zhao H, Shang Y, Wen Y, Lin Y, Zhou T, Zhou Z, Dong W, Hu Z, Guo X, Sha J, Li W*. Yeast model identifies ENTPD6 as a potential non-obstructive azoospermia pathogenic gene. Sci Rep. 2015 Jul 8;5:11762. doi: 10.1038/srep11762.
  46. Song ZH, Yu HY, Wang P, Mao GK, Liu WX, Li MN, Wang HN, Shang YL, Liu C, Xu ZL, Sun QY, Li W*, Germ cell specific Atg7 knockout results in primary ovarian insufficiency in female mice. Cell Death Dis.  2015 Jan 15;6:e1589. doi: 10.1038/cddis.2014.559.
  47. Liu WX, Shang YL, Li W*. gp78 G2BR and CUE domains cooperatively elongate polyubiquitin chains from the distal end. Sci. Rep. 2014 Nov 20;4:7138. doi: 10.1038/srep07138.
  48. Wang HN, Wan HF, Li XX, Liu WX, Chen Q, Wang YQ, Yang L, Duan EK, Tang HM, Zhang XJ, Zhao XY, Gao F*, Li W*. Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res. 2014 Jul;24(7):852-69.
  49. Li Y, Wu H, Wu W, Zhuo W, Liu W, Zhang Y, Cheng M, Chen YG, Gao N, Yu H, Wang L*, Li W*, Yang M*. Structural insights into the TRIM family of ubiquitin E3 ligases. Cell Res. 2014 Jun; 24(6):762-5.
  50. Chen M, Wang X, Wang Y, Zhang L, Xu B, Lv L, Cui X, Li W*, Gao F*. Wt1 is involved in leydig cell steroid hormone biosynthesis by regulating paracrine factor expression in mice. Biol Reprod. 2014 Apr 3;90(4):71, 1-9
  51. Yan L, Liu WX, Zhang HH, Liu C, Shang YL, Ye YH, Zhang XD*, Li W*, Ube2g2-gp78-mediated Herp polyubiquitination is involved in ER stress recovery. J Cell Sci. 2014 Apr 1;127: 1417-27.
  52. Liu WX, Shang YL, Zeng Y, Liu C, Li YC, Zhai LH, Wang P, Lou JZ, Xu P, Ye Y*, Li W*. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains. EMBO J. 2014 Jan 1;33(1):46-61.
  53. Qian MX, Pang Y, Liu CH, Haratake K, Du BY, Ji DY, Wang GF, Zhu QQ, Song W, Yu Y, Zhang XX, Huang HT, Miao S, Chen LB, Zhang ZH, Liang YN, Liu S, Cha H, Yang D, Zhai Y, Komatsu T, Tsuruta F, Li H, Cao C, Li W, Li GH, Cheng Y, Chiba T, Wang L, Goldberg AL, Shen Y, Qiu XB. Acetylation-mediated proteasomal degradation of core histones during DNA repair and spermatogenesis. Cell. 2013 May 23;153 (5):1012-24.
  54. Cui L, Li W*. Role of ubiquitination in meiotic recombination repair. Sci China Life Sci. 2010,53 (4): 447-454.
  55. Li W#, Tu D#, Li LY, Wollert T, Ghirlando R, Brunger AT, Ye Y. Mechanism of ubiquitin chain assembly on the active site cysteine of the ubiquitin conjugating enzyme Ube2g2. Proc Natl Acad Sci U S A. 2009 Mar 10; 106(10):3722-7.
  56. Li W and Ye Y (2008) Polyubiquitin chains: functions, structures, and mechanisms. Cell Mol Life Sci 2008 Apr 26. 65(15): 2397-406
  57. Li W, Tu D, Brunger A, Ye Y. (2007) A ubiquitin ligase transfers preformed polyqubiquitin chains from a conjugating enzyme to substrate. Nature 446(7133):333-7.
  58. Tu D, Li W, Ye Y, Brunger A. Structure and function of the yeast U-box-containing ubiquitin ligase Ufd2p. Proc Natl Acad Sci U S A. 2007 104(40):15599-606.
  59. Li W#, Sun L#, Liang Q#, Wang J, Mo W, Zhou B. Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging. Mol Biol Cell. 2006 Apr;17(4):1802-11.
  60. Li W, Mo W, Shen D, Sun L, Wang J, Lu S, Gitschier JM, Zhou B. Yeast model uncovers dual roles of mitochondria in action of artemisinin. PLoS Genet. 2005 1(3):e36
#共同第一作者, *通讯作者

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