Location: Home > Scientists
  Principal Investigators
Name:
 WANG Haoyi
Subject:
 Development Biology
Tel/Fax:
 +86-10-82619213  / 
E-mail:
 wanghaoyi@ioz.ac.cn
Address:
 The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences,1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China
More:
 Group of Genome Engineering Technology      
Resume:

Dr. Wang has an interdisciplinary training in genetics, molecular biology, and stem cell biology. As a doctoral and post-doctoral researcher, Dr. Wang has worked on the development of a variety of genome engineering technologies, including transposon-based “Calling Card” method for determining the genome-wide binding locations of transcription factors, TALEN-mediated genome editing in human pluripotent stem cells and mice, CRISPR-mediated multiplexed genome editing in mice, and CRISPR-mediated gene activation in human cells.

 

In 2014, he established his own lab at the Institute of Zoology, Chinese Academy of Sciences (Beijing, China). Since then, Wang lab has developed Zygote Electroporation of Nuclease (ZEN) method to generate genetically modified mouse models with high throughput and efficiency, Casilio method to regulate gene transcription, as well as method to generate CAR-T cells with multiplex gene editing. Wang laboratory focuses on developing novel technologies to achieve efficient and specific genome engineering, and applying them to study the function of genes and establish novel therapeutic methods.


2014 – present:

Principle Investigator, Group leader of Genome Engineering Technology

2009– 2014:

Postdoctoral Associate with Rudolf Jaenisch, Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

Development and application of site-specific nucleases on genome engineering of hES,hiPS cells and mouse.

2003–2009:

Doctoral Candidate, with Mark Johnston and Rob Mitra, Washington University in St. Louis.

Development and application of transposon “calling cards”, a novel method for determining thegenome-wide binding locations of transcription factors.

2001–2003:

Masters Candidate, Laboratory of Dr. Susan Hoffman, Miami University.

Detailed analysis of the organization and evolution of the Cyp2 gene family cluster in mouse.

1997 – 2010:

B.S. Candidate, Biology, Xiamen University, Fujian, China

Research Interests:
1. Developing novel tools for genome engineering
2. Establishment of novel therapeutic methods using gene editing
3. Study the mechanism of X inactivation in human using stem cell and？CRISPR technologies

Awards and Honors:

Professional Activities:

Research Grants:

Selected Publications:
  1. Li N*, Tang N, Cheng C, Hu T, Wei X, Han W, Wang H#. “Improving the anti-solid tumor efficacy of CAR-T cells by inhibiting adenosine signaling pathway.” OncoImmunology, 2020 Sep 24 Online.
  2. Sun W*, Wang H#. (2020). “Recent advances of genome editing and related technologies in China”. Gene Therapy, 2020 Aug; 27(7-8): 312-320. 
  3. An C*, Feng G*, Zhang J, Cao S, Wang Y, Wang N, Lu F, Zhou Q, Wang H#. “Overcoming Autocrine FGF Signaling-Induced Heterogeneity in Naive Human ESCs Enables Modeling of Random X Chromosome Inactivation.” Cell Stem Cell, 2020 Sep; 27（3）: 482-497.e4. 
  4. Tang N*, Cheng C*, Zhang X, Qiao M, Li N, Mu W, Wei X, Han W, Wang H#. “TGF β Inhibition via CRISPR Promotes the Long-Term Efficacy of CAR-T Cells Against Solid Tumors.” JCI Insight, 2020 Feb; 5(4): e133977. 
  5. Cao Y*, Sun W*, Wang J, Sheng G, Xiang G, Zhang T, Shi W, Li C, Wang Y, Zhao F#, Wang H#. “Argonaute proteins from human gastrointestinal bacteria catalyze DNA-guided cleavage of single- and double- stranded DNA at 37 °C.” Cell Discovery, 2019; 5:38.
  6. Wang H#，Yang H#. “Gene-edited babies: What went wrong and what could go wrong.” PLOS Biology, 2019 Apr 30 Online.
  7. Mu W*, Tang N*, Cheng C*, Sun W, Wei X, Wang H#. “In vitro transcribed sgRNA causes cell death by inducing interferon release.” Protein & Cell, 2019 Jan 7 Online. 
  8. Cheng C*, Tang N*, Li J, Cao S, Zhang T, Wei X, Wang H#. “ Bacteria-free minicircle DNA system to generate integration-free CAR-T cells.” Journal of Medical Genetics, 2019 Jan; 56(1): 10-17. 
  9. Xiang G*, Ren J*, Hai T*, Fu R, Yu D, Wang J, Li W, Wang H#, Zhou Q#. “Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs.”  Cellular and Molecular Life Sciences. 2018 Dec; 75(24): 4619-4628.  
  10. Xiang G, Wang H#. “Extended pluripotent stem cells facilitate mouse model generation.”  Protein & Cell. 2018 Aug 21. doi: 1007/s13238-018-0573-0.
  11. Mu W, Zhang Y, Xue X, Liu L, Wei X, Wang H#.” 5′ capped and 3′ polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system.” Protein & Cell, 2018. June Online. 
  12. Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H#.“CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells.” Frontiers of Medicine, 2017 Dec;11(4):554-562.
  13. Xiang G, Zhang X, An C, Cheng C, Wang H#.“Temperature effect on CRISPR-Cas9 mediated genome editing.”Journal of Genetics and Genomics, 2017 Apr;44(4):199-205.
  14. Liu X, Zhang Y, Cheng C, Cheng WA, Zhang X, Li N, Xia C, Wei X, Liu X, Wang H#. “CRISPR-Cas9 mediated multiplex gene editing in CAR-T cells.” Cell Research, 2017 Jan;27(1):154-157.
  15. Burgess S, Cheng L#, Gu F, Huang J, Huang Z, Lin S#, Li J, Li W, Qin W, Sun W, Songyang Z, Wei W#, Wu Q, Wang H#, Wang X, Xiong JW, Xi J, Yang H, Zhou B, Zhang B. “Questions about NgAgo” Protein Cell, 2016 Dec;7(12):913-915.
  16. Wang W,Kutny PM, Byers SL, Longstaff CJ, DaCosta MJ, Pang C, Zhang Y, Taft RA, Buaas FW, Wang H#. “Delivery of Cas9 protein into mouse zygotes through a series of electroporation dramatically increased the efficiency of model creation.” Journal of Genetics and Genomics, 2016 May 20;43(5):319-27.
  17. Qin W, Kutny PM, Maser RS, Dion SL, Lamont JD, Zhang Y, Perry GA, Wang H#.“Generating Mouse Models Using CRISPR-Cas9 Mediated Genome Editing.” Current Protocols in Mouse Biology, 2016 Mar 1;6(1):39-66. 
  18. Cheng AW*#, Jillette N*, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H#. “Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling.” Cell Research, 2016 Feb;26(2):254-7. 
  19. Wiles MV, Qin W, Cheng AW, Wang H#. “CRISPR-Cas9 mediated genome editing and guide RNA design.” Mammalian Genome, 2015 Oct;26(9-10):501-10.
  20. Qin W, Dion SL, Kutny PM, Zhang Y, Cheng AW, Jillette NL, Malhotra A, Geurts AM, Chen YG, Wang H#. “Efficient CRISPR/Cas9-mediated genome editing in mice by zygote electroporation of nuclease.” Genetics, 2015 Jun;200(2):423-30.
  21. Theunissen T*, Powell B*, Wang H*, Mitalipova M, Faddah D, Reddy J, Fan Z, Maetzel D, Ganz K, Shi L, Lungjangwa T, Imsoonthornruksa S, Stelzer Y, Rangarajan S, D’Alessio A, Zhang J, Gao Q, Dawlaty M, Young R, Gray N, Jaenisch R. “Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency.”Cell Stem Cell, 2014 Oct 2; 15(4):471-87.
  22. Yang H, Wang H, Jaenisch R. “Generating genetically modified mice using CRISPR/Cas-mediated genome engineering.” Nature protocols, 2014 Aug; 9(8):1956-68.
  23. Maetzel D*,Sarkar S*, Wang H*, Mosleh LA, Cheng AW, Xu P, Gao Q, Mitalipova M, Jaenisch R. “Genetic and chemical correction of cholesterol accumulation and impaired autophagy in hepatic and neural cells derived from Niemann-Pick Type C patient-specific iPS cells.” Stem Cell Reports, 2014 May 15; 2(6):866-80.
  24. Cheng AW*, Wang H*, Yang H, Shi L, Katz Y, Rangarajan S, Theunissen TW, Shivalila CS, Dadon DB, Jaenisch R. “Multiplexed activation of endogenous genes by CRISPR-on, a RNA-guided transcriptional activator system.” Cell Research, 2013 October 1; 23(10): 1163-1171.
  25. Yang H*, Wang H*, Shivalila CS*, Cheng AW, Shi L, Jaenisch R. “One-step generation of mice carrying reporter and conditional allele by CRISPR/Cas mediated genome editing” Cell, 2013 September 12; 154(6):1370-1379.
  26. Faddah D, Wang H, Buganim Y, Cheng AW, Jaenisch R. “Expression of Nanog is biallelic and equally variable as other pluripotency factors.” Cell Stem Cell, 2013 Jul 3;13(1):23-9.
  27. Wang H*, Hu YC*, Markoulaki S, Welstead GG, Shivalila CS, Cheng AW, Pyntikova T, Dadon D, Voytas DF, Bogdanove AJ, Page DC, Jaenisch R. “TALEN-mediated editing of the Mouse Y Chromosome.” Nature Biotechnology, 2013 Jun; 31(6):530-2.
  28. Wang H*, Yang H*, Shivalila CS*, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. “One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas mediated genome engineering.” Cell, 2013 May 9;153(4):910-8. 
*These authors contribute equally to this work.